Table 4 Standard curve characteristics for quantitative polymerase chain reaction (qPCR) analyses performed by the U.S. Geological Survey Ohio Water Microbiology Laboratory during this study of Kabetogama Lake Sullivan Bay, Northwest, near Ash River (U.S. Geological Survey Site No. 482542092493701), Voyageurs National Park, MN, USA, 2016.

From: Phytoplankton community and algal toxicity at a recurring bloom in Sullivan Bay, Kabetogama Lake, Minnesota, USA

qPCR assay

Dynamic range

Amplification efficiency (percent)

R2 value

LoD

LoQ

general cyanobacteria

66.0–6.60E + 07

95–97

0.995–0.996

26

72

general Microcystis

9.44–9.44E + 06

91–96

0.994–0.997

27

55

general Planktothrix

16.1–1.61E + 07

99–100

0.998–0.999

18

34

general Dolichospermum

24.6–2.46E + 07

98–100

0.992–0.995

230

550

Microcystis microcystin toxin gene (mcyE)

11.4–1.14E + 07

93–98

0.997

13

39

Planktothrix microcystin toxin gene (mcyE)

28.4–2.84E + 07

94–95

0.993–0.998

51

110

Dolichospermum microcystin toxin gene (mcyE)

19.2–1.92E + 07

95–96

0.997–0.998

46

120

saxitoxin toxin gene (sxtA)

30.8–3.08E + 07

81–84

0.995–0.998

14

45

anatoxin-a toxin gene (anaC)

74.5–7.45E + 07

83–84

0.999

32

60

  1. [qPCR, quantitative polymerase chain reaction; dynamic range, limit of detection, and limit of quantification are reported in copies per reaction; R2, coefficient of determination; LoD, limit of detection; LoQ, limit of quantification].
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