Figure 1

Leukopenic toxicity and body weight loss after influenza vaccine injection. Mice were intraperitoneally injected with 0.5 mL of each influenza vaccine. The concentrations of the toxicity reference vaccine (RE) were 0.0625, 0.25, or 1.0 U/mouse. The dose of hemagglutinin split vaccine (HAV) was 0.9375, 3.75, or 15 µg hemagglutinin (HA)/0.5 mL. Sterilized physical saline (SA) was used as the control. Sixteen hours after vaccination, blood was collected to assess the number of leukocytes. The animal experimental protocol used in this study is shown in (a). The body weight change was calculated by subtracting the weight before vaccination from the weight 16 h after vaccination. The data shown are (b) the number of leukocytes (indicated as white blood cells, [WBC]) and (c) the body weight changes. Each plot indicates the individual animals, and the error bar indicates the standard deviation (SD). **p < 0.01 and ***p < 0.001 (n = 5 in each group).