Figure 6
Principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots derived from the metabolite concentrations in urine obtained from mice treated with sterilized physical saline (SA), 15 μg hemagglutinin (HA)-containing hemagglutinin split vaccine (HAV), or 1.0 U of the toxicity reference vaccine (RE). (a) OPLS-DA score plots derived from the 1H NMR spectra of urine from the SA-, HAV-, or RE-treated groups. Each PCA model was generated with principal components (PC), and each OPLS-DA model was generated with predictive components (T) and orthogonal components (TO) to discriminate between groups. (b) OPLS-DA loading plot of the mouse urine samples obtained from the SA-, HAV-, or RE-treated groups. Each dot in the loading plot represents a metabolite that was identified as one of the most influential variables according to its respective contributions to the discrimination of the groups; red dots indicate potential biomarkers. (c) OPLS-DA loading column plot of the discriminant variables. Red columns indicate potential biomarkers for RE.
