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Figure 1

From: Robust Preimplantation Genetic Testing of Huntington Disease by Combined Triplet-Primed PCR Analysis of the HTT CAG Repeat and Multi-Microsatellite Haplotyping

Figure 1

Comparison of standard PCR and TP-PCR for determination of HTT CAG alleles. Four cell lines (GM07175, GM04282, GM05539 and GM09197) representing different allele ranges were used and their genotypes are indicated at the top of each column. Standard PCR and TP-PCR were compared at genomic DNA and single cell levels, including direct single cell PCRs and whole genome amplified single cell PCRs. Asterisks denote presence of fluorescent peaks that were too low to be sized by the genetic analyzer. ADO, allele drop-out.

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