Figure 4

Repression of P2rx1 by progressive vascular endothelial damage. (a–c) RQ-PCR (a) and western blot (b,c) analysis of the expressions of the cholinergic receptor muscarinic 2 (Chrm2), cholinergic receptor muscarinic 3 (Chrm3), and purinergic receptor P2X 1 (P2rx1) genes in the bladders of rats in the indicated groups. Expression levels of the indicated transcripts are presented as % Gapdh. For western blot analysis, β-actin was used as a loading control, and the expression levels of the indicated proteins were normalized to the β-actin value. (d,e) Representative images (d) and quantification analysis (e) of the immunohistochemical staining for the P2rx1 protein (original magnification ×200, scale bar = 200 μm). All quantification results are presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001, compared to the sham group; ^###p < 0.05, ^###p < 0.01, ^###p < 0.001, compared with the AI-30 group according to the unpaired t-test (c) or one-way analysis of variance with the Bonferroni post hoc test. (a–e) Source data with exact p-values and number of replicates can be found in the Supplementary Dataset.