Figure 1
From: Mild chronic perturbation of inhibition severely alters hippocampal function
GABA responses from N2a cells expressing WT γ2 or γ2*, with WT α1 and β2. (A) GABA responses (100 μM) ± 100 µM PTX from representative WT and γ2* expressing cells. (B) Summary of 8 cells. *P = 0.013 by Welch’s t test. (C) Responses from increasing concentrations of GABA (1, 3, 10, 30, and 100 μM) from representative WT and γ2* expressing cells in the absence of PTX (N2a cells). (D) Dose-response curves of WT (n = 9) and γ2* (n = 10) expressing cells, fit with the Hill equation and yielding similar EC50 values (see text). (E) Reversal potential of WT and γ2* receptors obtained with a cesium methanesulfonate solution as detailed in the Methods.
