Figure 1

Identification of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) in the mudskipper. (a) The amino-acid sequences of the mature forms of ANP and BNP deduced from cloned cDNA sequences. Highlighted residues are identical to those in the mudskipper sequences. Disulfide bonds are indicated by brackets. (b) Tissue distribution of anp and bnp mRNAs. Elongation Factor 1 α (ef1α) mRNA was used as a control for each cDNA sample. Reverse transcription control without template was not detected in any tissue. The gel pictures were cropped and their full-length gels are shown in Supplementary Fig. 2.