Figure 7
CD spectra of cell lysates prepared from β2-AR-transfected/untransfected HEK-293 cells incubated with different concentrations of P3 in the presence or absence of the agonist isoproterenol. Lysates of HEK-293 cells transiently transfected with β2-AR/Rluc8 (a and b) and mock-transfected (control) HEK-293 cells (c) were titrated with P3 in the presence (a,c) or absence of the β2-AR agonist isoproterenol (b). In the presence of agonist, the cell lysate was preincubated with 1 µM isoproterenol, and then P3 was added at a final concentration of 5, 10, 20 or 30 µM. The CD spectrum of P3 (P3, dark blue line) in buffer is shown for comparison. All experiments were performed in PBS, pH 7.2, containing 0.1% DMSO at 25 °C.
