Table 3 Primers of qPCR used in the experiment.
Genes | Sequences (5ʹ-3ʹ) | Product size | Tm (°C) | |
|---|---|---|---|---|
Heat shock protein kDa70, HSP70 | F: AAGGCAGAGGATGATGTC R: TGCAGTCTGGTTCTTGTC | 186 | 59 | Mohd-Shaharuddin, et al.86 |
Heat shock protein kDa90, HSP90 | F: ACCTCCCTCACAGAATACC R: CTCTTGCCATCAAACTCC | 197 | 59 | Mohd-Shaharuddin, et al.86 |
Complement 3, C3 | F:GCAATCCTCCACAACTACAG R: ACTCTGACCTCCTGACGATAC | 11 | 59 | Mohd-Shaharuddin, et al.86 |
Complement 4, C4 | F: TTGCTTCTTCCCTACAGTG R: GGTCCAACCCTCCTTTAC | 185 | 59 | Mohd-Shaharuddin, et al.86 |
MHC class IIb, MHC-IIb | F: GTTGGATACACTGAGTTTGG R: GAGGGTTTGACTGACTTAGAC | 152 | 60 | Mohd-Shaharuddin, et al.86 |
18S rRNA, 18S | F:TGGTTAATTCCGATAACGAACGA R: CGCCACTTGTCCCTCTAAGAA | 94 | 59/60 | Mohd-Shaharuddin, et al.86 |
Elongation factor-1α, ef1α | F: AAATTGGCGGTATTGGAAC R:GGGAGCAAAGGTGACGAC | 83 | 59/60 | Mohd-Shaharuddin, et al.86 |
