Figure 6
H&E and immunohistochemistry of ferret lung and brain. Representative H&E (a,c,e,g,i,k) and immunohistochemistry labeled with a NiV N protein-specific polyclonal rabbit antibody (b,d,f,h,j,l). Lung (a,b,e,f,i,j) and brain (c,d,g,h,k,l) from representative ferrets infected with rNiVM-wt (a–d), rNiVM-PY116E (e–h), and rNiVM-PΔ116-135 (i–l). White arrow points to area of marked locally extensive, vacuolar plaque with necrosis and gliosis within the grey matter in regions with diffuse, strongly immunopositive neuronal involvement. No vacuolar plaque is observed without associated NiV antigen in the frontal cortex of rNiVM-PY116E-02. Images taken: lung 20×, brain 20×. Normal ferret histology for these tissues can be seen in ref.20.
