Table 1 Clinical disease in ferrets after experimental infection with rNiV_M.

rNiV_M-wt-1

EU

d8

+

+

+

d4-7

Thrombocytopenia (d6,8); lymphopenia (d6); hypoalbuminemia (d6,8); >3-fold increase in BUN (d8); depression (d5-7); lethargy (d5-7); inappetence (d6-8); dehydration (d8); rales (d6-8); ocular, nasal, and oral discharge (d6); ataxia (d6-8); severe hypothermia (d8).

rNiV_M-wt-2

EU

d7

+

++

−

d4-6

Thrombocytopenia (d6,7); hypoalbuminemia (d7); hyperglycemia (d7); depression (d6-7); lethargy (d6-7); inappetence (d7); ocular and nasal discharge (d6-7); myoclonus (d7); ataxia (d7).

rNiV_M-wt-3

EU

d8

+

+

+

d4-6

Thrombocytopenia (d6,8); lymphopenia (d8); hypoalbuminemia (d6,8); >3-fold increase in BUN (d8); depression (d6-8); lethargy (d6-8); inappetence (d7-8); sneezing (d7-8); nasal discharge (d6-8); rales (d6-8); ataxia (d7-8); obtunded (d8); hypothermia (d8); loss of >10% body weight.

rNiV_M-wt-4

EU

d6

+

+

−

d5-6

Thrombocytopenia (d3,6); lymphopenia (d6); hypoalbuminemia (d6); depression (d6); lethargy (d6); nasal discharge (d6); myoclonus (d6); hypothermia (d6).

rNiV_M-wt-5

EU

d7

+

++

−

d4-6

Thrombocytopenia (d3,6,8); lymphopenia (d6); hypoalbuminemia (d6-7); >3-fold increase in BUN (d7); hyperglycemia (d6,7); depression (d6-7); lethargy (d6-7); inappetence (d6-7); ocular and nasal discharge (d7); rales (d7); myoclonus (d7); ataxia (d7); hindlimb paresis (d7); hypothermia (d7); loss of >10% body weight.

rNiV_M-P_Y116E-1

EU

d8

+

+++

+

d5-7

Thrombocytopenia (d8); lymphopenia (d8); hypoalbuminemia (d6,8); hyperglycemia (d8); depression (d7-8); lethargy (d7-8); inappetence (d7-8); nasal discharge (d7-8); myoclonus (d8); seizures (d8); loss of >10% body weight.

rNiV_M- P_Y116E -2

EU

d8

+

+++

−

d6-8

Thrombocytopenia (d8); lymphopenia (d8); hypoalbuminemia (d8); >3-fold increase in BUN (d8); hyperglycemia (d8); depression (d7-8); lethargy (d7-8); oral and nasal discharge (d6-8); rales (d8); aggressive behavioral change with vocalization and visual deficit (d8); ataxia (d8); seizures (d8).

rNiV_M- P_Y116E -3

EU

d9

+

++

+

d7-9

Thrombocytopenia (d9); lymphopenia (d9); hypoalbuminemia (d9); hyperglycemia (d9); depression (d7-9); lethargy (d7-9); rales (d8-9).

rNiV_M- P_Y116E -4

EU

d9

+

+

+

d6-8

Thrombocytopenia (d9); lymphopenia (d9); hypoalbuminemia (d9); hyperglycemia (d9); depression (d7-9); lethargy (d8-9); nasal discharge (d6-9); rales (d8-9).

rNiV_M- P_Y116E -5

EU

d8

+

+++

+

d5-8

Thrombocytopenia (d8); lymphopenia (d6,8); hypoalbuminemia (d8); hyperglycemia (d8); depression (d7-8); lethargy (d7-8); nasal discharge (d6-8); hindlimb paresis (d8); seizures (d8).

rNiV_M-P_Δ116-135-1

EU

d11

−

+++

+

d6-9

Thrombocytopenia (d6,10,11); lymphopenia (d6,10); >3-fold increase in BUN (d11); hyperglycemia (d8,11); depression (d6-8); lethargy (d6-8); inappetence (d10); left hindlimb paresis (d10); quadraparesis (d11); hypothermia (d12); loss of >10% body weight.

rNiV_M-P_Δ116-135-2

EU

d12

+

+++

−

d6-11

Thrombocytopenia (d10,12); lymphopenia (d10); depression (d6-8); lethargy (d6-8); inappetence (9-10); sneezing (d9-10); nasal discharge (d10); facial and cervical tremors (d11-12); myoclonus (d11-12); hindlimb paresis (d12); hypothermia (d12); loss of >10% body weight.

rNiV_M- P_Δ116-135-3

EU

d12

+

+++

−

d6-12

Thrombocytopenia (d12); lymphopenia (d6); depression (d6-8); lethargy (d6-8); inappetence (d9); seizures (d12).

rNiV_M- P_Δ116-135-4

EU

d12

+

+++

+

d6-11

Thrombocytopenia (d10,12); lymphopenia (d6); hypoalbuminemia (d8); >3-fold increase in BUN (d12); hyperglycemia (d8); depression (d6-8); lethargy (d7-8); inappetence (d9); sneezing (d9-10); myoclonus (d10-12); quadraparesis (d12); severe hypothermia (d12); loss of >10% body weight.

rNiV_M- P_Δ116-135-5

EU

d12

+

+++

−

d5-12

Thrombocytopenia (d10,12); lymphopenia (d12); hypoalbuminemia (d8,12); depression (d6-8); lethargy (d7-8); inappetance (d9-10); sneezing (d9); nasal discharge (d9); myoclonus (d11); hindlimb paresis (d12); loss of >10% body weight.