Table 3 Polymerase chain reaction primers and annealing temperatures used to amplify and sequence selected mRNA transcripts of warthogs.

From: Cytokine gene expression assay as a diagnostic tool for detection of Mycobacterium bovis infection in warthogs (Phacochoerus africanus)

Gene

Forward (5′-3′)

Reverse (5′-3′)

Tannealing

Accession numbers

B2M

ATTCCACCGCCAGCACCGCT

CCCCCTCTACATCTACCTGCT

56 °C

MK333445

H3F3A

ATGGCYCGWACMAAGCAGAC

CGRCGWGCYARCTGGATGTC

56 °C

MK333454

LDHA

GAAGTGCACTCCCGATTCCT

AGGCTGTCTTAACATTACTGCT

56 °C

MK333450

PPIA

TCGTGCTGCCTTGCA

GCTACAGAAGGAATGGTCTG

51 °C

MK333451

YWHAZ

RCASAACATCCAGTC

AARTGGTCTACTGTGTAAAT

45 °C

MK333453

CXCL9

ACAGRAGTGAYWYYRYYCTACCA

GCCMTCCYYTTYWGGAATTATTTCAG

55 °C

MK333446

CXCL10

CAKTSKGAGCCTRCMGCAGAAG

ARTCCAYGGACADTTAGGGCTTSA

57 °C

MK333447

CXCL11

TACTCCCTCCAAGAAGAGTATCA

AGCGTTCTTATTTCAGTATTCACAGT

53 °C

MK333448

IFNG

TCTGGGCCTGATCGACTGTA

TTTGATCAATGAATCAATATTCCCCA

52 °C

MK333449

TNFA

ACYTGARCCCYTCTGAAAA

AAACCAGAAGGRSRTGAG

50 °C

MK333452

  1. The NCBI accession number of each mRNA sequence is listed.
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