Figure 2
Total 5-HT2CR protein levels do not differ between the Cys23- and Ser23-CHOp38 cell lines. (A) Representative saturation binding isotherm of stably transfected Cys23 and Ser23 5-HT2CR-CHOp38 cells. Results obtained for KD and Bmax values were from 3 independent experiments. Total homogenate (B) 5-HT2CR (n.s.; n = 7), (C) TfR (n.s.; n = 3) and (D) p38 (n.s.; n = 6) protein expression normalized to β-actin in stably expressing Cys23 and Ser23 5-HT2CR-CHOp38 cells. Representative virtual blot-like images (left) and electropherograms adjusted to baseline (right) were generated by the Simple Western Compass software using the area under the curve for the peak of interest. Full length electropherograms and virtual blot-like images are located in Supplementary Figure 2.
