Figure 4
GalNac-O-Bn treatment decreases colocalization of the Ser23 allele with GRASP65 with no effect on the Cys23 allele. Colocalization of transiently transfected CHOp38 cells expressing (A) Cys23 or (B) Ser23 5-HT2CR in green with the Golgi reassembly-stacking protein of 65 kDa (GRASP65) in red treated with vehicle or 2 mM Benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (GalNAc-O-Bn) and represented as a single mid-cell slice. The scatter plot represents the type and intensity of pixels for the evaluated cell. Scale bar = 10 µm. Pearson’s Correlation between the 5-HT2CR and the GRASP65 for (C) Cys23 and (D) Ser23 CHOp38 cells. Each experiment was performed on four to eight biological replicates with 20–30 fields of view per experiment and 40–70 cells analyzed for each condition per receptor variant. *p < 0.05 vs. Vehicle.
