Figure 5

Results of the melting temperature (Tm) measurements for the untreated (panel a) and treated (panel b) apo- and holo- Cv-ATA wild-type (WT), Y322A and K288A. The values for the untreated samples are shown as absolute values of Tm expressed in °C. The values for the treated samples (either reduced with the reducing agent NaCNBH3 or incubated in the presence of amino donor L-alanine to form non-covalently coordinated PMP) are reported as Tm shifts (ΔTm) expressed in Δ°C.