Table 1 Stereological analysis of Shh reporter expression in different cell populations in the dentate gyrus.

From: Sonic Hedgehog is expressed by hilar mossy cells and regulates cellular survival and neurogenesis in the adult hippocampus

1.Label*

2.Cell Type

3.No. cells label

4.Label/LacZ

5.Colocalization

% Label/LacZ

6.total LacZ

7.Colocalization

%LacZ/Label

8.No. mice

X slice

PLP

Oligodendrocytes

760 ± 38.9

0

0

n.q

0

3x10

GFAP

Astrocytes

1013.3 ± 83.7

0

0

n.q

0

7x10

NeuN

Neurons

3834 ± 320

1120 ± 51.1

29.2 ± 1.3

1138.2 ± 56

98.4 ± 5.0

3x10

CR

GABA subtype

1574.3 ± 62.4

1140 ± 62.4

72.4 ± 3.9

1162.1 ± 91.2

98.1 ± 8.4

7x10

SST

GABA subtype

1062.5 ± 113.3

0

0

n.q

0

3x10

PV

GABA subtype

528.6 ± 69.9

0

0

n.q

0

3x10

NPY

GABA subtype

462.5 ± 40

0

0

n.q

0

3x10

GAD 65/67

GABAergic

3433.3 ± 391.3

1114.3 ± 94.9

32.4 ± 2.8

1146.1 ± 81.3

97.2 ± 7.3

7x10

GlutR2/3

Glutamatergic

3116.7 ± 188.8

1300.2 ± 77.5

41.7 ± 5.0

1370 ± 87.9

92.4 ± 9.2

6X10

  1. The Shh-lacZ reporter was expressed in only in neural phenotypes NeuN, GAD 65/67,CR and GluR2/3 and CGRP. This analysis leads to conclude that Shh is exclusively express in mossy cells that express CR and GAD. % Cells labeled for a cell type that express lacZ (column 5) and, conversely, lacZ+ cells that express a cell type label (column 7) were quantified. (n.q.): lacZ cells were “not quantified” (column 6) when the reporter was absent in the cell type of interest (column 4). The number of cells were estimated stereologically13 (see methods). *PLP (proteolipid protein), GFAP (glial fibrillary acidic protein), NeuN (neuronal nuclei), CR (calretinin), SST (somatostatin), PV (parvalbumin), NPY (neuropeptide Y), GAD (glutamic acid decarboxylase), GluR2/3 (Glutamatergic receptor 2 and 3).
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