Figure 3

Data quality control. (A) Venn diagram showing the number of quantified proteins detected in two biological replicates from six different 2D and 3D mono-/co-cultured 3T3-L1 adipocytes with macrophages. The intersection shows a total of 2,885 proteins (83.6%) quantified in both biological replicates. (B) Diverse log2 iTRAQ ratios of six different 3T3-L1 cells were automatically normalized using Proteome Discoverer 1.4.1.14.