Table 1 Sequences of the primers used in this study.

From: The VP3 protein of duck hepatitis A virus mediates host cell adsorption and apoptosis

Gene

Forward primer

Reverse primer

VP0

5′-CCATCTGTGTCATTGTGTTAGGCA-3′

5′-CAAATCAGTTTCAAGGAGTTCTCCA-3′

Caspase-3

5′-TGGTGTTGAGGCAGACAGTGGA-3′

5′-CATTCCGCCAGGAGTAATAGCC-3′

Caspase-8

5′-GGTGATGCTCGTCAGAAAGGTG-3′

5′-AGCCATGCCCAAGAGGAAGT-3′

Caspase-9

5′-GCTGCTTCAACTTCCTCCGTAA-3′

5′-CATCTCCACGGACAGACAAAGG-3′

β-Actin

5′-CCGGGCATCGCTGACA-3′

5′-GGATTCATCATACTCCTGCTTTGCT-3′

VP3

5′-CATCATTTTGGCAAAGAATTCGCCAC CATGGGAAAGAGAAAACCATGCAGG-3′

5′-TTGGCAGAGGGAAAAAGATCTTCAC TTATCGTCGTCATCCTTGTAATCTTGATTGTTAGTTGCCATCTGC-3′

  1. Note: The VP3 forward primer included the 15 bp upstream vector terminal homologous sequence (indicated by the solid line below) and an EcoRI restriction site (shown in the box). The 5′ terminus of the reverse primer included the 15 bp downstream vector terminal homologous sequence (shown under the solid line), a Bgl II cleavage site (shown in the box) and a FLAG tag (indicated by the wavy line).