Figure 1
From: PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease
Effect of PD-1 blockade on functional suppression of post-EAU Treg cells. Spleen cells from post-EAU mice were collected and reactivated in vitro with IRBP and adoptively transferred to recipient mice immunized for EAU. The solid line with closed circles are EAU control mice that did not receive an adoptive transfer of spleen cells, the dashed line with open squares indicates mice were immunized for EAU and received spleen cells. One experimental group of mice received post-EAU spleen cells alone (A, n = 8), and another experimental group of mice received post-EAU spleen cells that were reactivated with α-PD-1 blocking antibody (B, n = 15). Each experiment consisted of 4–5 mice, and each experiment was repeated 2–3 times. Significance was determined by two-way ANOVA with Bonferroni post-test.
