Figure 2
From: PD-1+ melanocortin receptor dependent-Treg cells prevent autoimmune disease
Effect of post-EAU Treg cells on the induction of suppressor APC. Spleen cells from post-EAU mice were collected and reactivated in vitro with IRBP, sorted, and incubated with APC from the spleen of unimmunized mice. Primed IRBP-specific T cells were collected from the popliteal lymph nodes and added to the APC from above, and transferred to recipient mice immunized for EAU. The solid line with closed circles represents EAU control mice that did not receive an adoptive transfer (AT) of cells. The dashed line with open squares indicates mice immunized for EAU that received cells. One group of mice received APC pre-incubated with T cells from unimmunized mice (A, n = 10), another group of mice received cells where APC were pre-incubated with sorted post-EAU Tregs (B, n = 10). Each experiment consisted of 5 mice, and each experiment was repeated 2 times. Significance was assessed by two-way ANOVA with Bonferroni post-test.
