Figure 1

(A) Close-up of active site of E. coli enolase showing residues interacting with the SF2312 inhibitor. Loop 1 is colored cyan and Loop 2 is colored magenta. (B) Ligplot schematics of interactions at active site in the SF2312-bound E. coli structure. (C) Superposition of the E. coli and human enolase dimer structures complexed with SF2312. (D) Close-up of active site showing Loop 3 (residues 248–268) in red for the E. coli structure. (E) Active site representation of the superposition of E. coli enolase in complex with SF2312 (cyan) and substrate 2-PGA (sand; PDB id code 6BFY). (F) Active site representation of the superposition of E. coli enolase (chain F) in complex with KSF (magenta) onto E. coli enolase with SF2312 (gray).