Figure 4 | Scientific Reports

Figure 4

From: Sorsby Fundus Dystrophy Mutation in Tissue Inhibitor of Metalloproteinase 3 (TIMP3) promotes Choroidal Neovascularization via a Fibroblast Growth Factor-dependent Mechanism

Figure 4

Mutant TIMP3 in RPE cells is an inefficient MMP inhibitor (a) Expression of TIMP3 protein was confirmed by western blot analysis in the ECM of RPE cells transfected with control vector (V), wild-type TIMP3 (W1) and S179C-TIMP3 (M1, M5). (b) MMP inhibitory activity of wild-type and mutant TIMP3 in the ECM of RPE cells was determined by reverse zymography (c) MMP2 and d) MMP9 levels were quantitated in the conditioned medium of RPE cells by ELISA. Data are expressed as means ± SD (n = 3). **p ≤ 0.01.

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