Figure 5 | Scientific Reports

Figure 5

From: Sorsby Fundus Dystrophy Mutation in Tissue Inhibitor of Metalloproteinase 3 (TIMP3) promotes Choroidal Neovascularization via a Fibroblast Growth Factor-dependent Mechanism

Figure 5

Increased bFGF in the CM of RPE cells expressing mutant TIMP3 is mediated by MMPs. ELISA was used to quantitate bFGF levels in the (a) CM and (b) ECM of RPE cells expressing control empty vector (V), wild-type TIMP3 (W1) or mutant TIMP3 (M1,M5) (c) Effect of an MMP2/9 inhibitor (SB-3CT, 5 μg/ml for16 hours) on bFGF secretion was evaluated in RPE cells expressing wild-type or mutant TIMP3. VEGF levels were quantitated in the (d) CM and (e) ECM of RPE cells expressing control empty vector (V), wild-type TIMP3 (W1) or mutant TIMP3 (M1,M5). Effect of MMP2/9 inhibitor (SB-3CT, 5 μg/ml for16 hours) on VEGF secretion was evaluated in RPE cells. Data are expressed as means ± SD (n = 3). **P ≤ 0.01 vs Vector.

Back to article page