Figure 1

Purification, assembly and disassembly of HIV-1 CANC. (a) Schematic representation of HIV-1 CANC protein and its full-length amino acid sequence; mutations used in this study are shown in red. (b,c) Coomassie Brilliant Blue -stained SDS polyacrylamide gels showing pooled fractions of HIV-1 CANC protein after purification by (b) cation-exchange and (c) gel filtration chromatography. (d) TEM analysis of negatively stained HIV-1 CANC protein under assembly/disassembly conditions. HIV-1 CANC protein was assembled in the absence (A) or presence (B–L) of tqON in assembly buffer containing 50 mM Tris, pH 8, 340 mM NaCl and 1 µM ZnCl₂. The indicated reactant (C–L) was added to the assembled tubular structures, and the incubation proceeded overnight at laboratory temperature.