Figure 2

Exonuclease activity and TEM analysis of CANC particles under assembly/disassembly conditions. Digestion of FAM-tqON by Exonuclease I in buffers with (a) different NaCl concentration and (b) pH. (c) TEM image of CANC assembled in presence of tqON in the assembly buffer (50 mM Tris, pH 7, 340 mM NaCl, 1 µM ZnCl₂). (d) TEM image of the CANC particles shown in (c) after incubation in the disassembly buffer (50 mM Tris, pH 7, 170 mM NaCl, 1 µM ZnCl₂).