Figure 3
From: A simple, high-throughput stabilization assay to test HIV-1 uncoating inhibitors
CANC disassembly monitored by DITH and TEM. (a) Fluorescence emission curves demonstrating the kinetics of tqON release from preassembled CANC particles incubated in assembly buffer (black curve) and disassembly buffer (red curve); the vertical bars represent standard deviations for single measured points. The relative stabilization was then calculated as the difference between the relative fluorescence of tqON after 90 min in the disassembly and assembly reactions (Δ1 in panel a). The stability of wt in assembly buffer was considered as 100%, the stability in disassembly buffer as 0%. (b,c)TEM analysis of negatively stained HIV-1 CANC protein preassembled in the presence of tqON in assembly buffer and then incubated in (b) assembly buffer or (c) disassembly buffer.
