Figure 1 | Scientific Reports

Figure 1

From: Correcting Artifacts in Single Molecule Localization Microscopy Analysis Arising from Pixel Quantum Efficiency Differences in sCMOS Cameras

Figure 1

sCMOS camera RQE maps and analysis artifacts in simulated data. (A) The measured gain for each pixel in a 64 by 64 pixel region of a sCMOS camera. (B) Same as (A) for a different sCMOS camera. (C) SMLM image resulting from the analysis of simulated SMLM movie. This image was created by rendering the localizations as Gaussians with unit height and a sigma equal to 1 pixel. A pixel itensity value of 420 corresponds to approximately 420 localizations found in or very near the pixel. The movie was analyzed assuming a constant RQE value of 1.0. The measured sCMOS calibration data shown in (A) was used to create the simulated SMLM movie as described in the main text. The image is 6.4 × 6.4 μm with a 100 nm pixel size (D) A scatter plot of the number of localizations identified in each pixel versus the pixels smoothed RQE value.

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