Figure 4 | Scientific Reports

Figure 4

From: Correcting Artifacts in Single Molecule Localization Microscopy Analysis Arising from Pixel Quantum Efficiency Differences in sCMOS Cameras

Figure 4

Images of mouse brain tissue stained with wheat germ agglutinin conjugated to ATTO-488, embedded in epoxy resin and sectioned at 70 nm thickness. (A) SMLM image of part of a tissue section analyzed without RQE correction showing evidence of a vertical striping artifact. (B) Same as (A) but analyzed with RQE corrected localization identification and fitting. (C) SMLM image of the same camera ROI shown in (A) but of a different physical tissue section. (D) Same as (C) but analyzed with RQE-corrected localization identification and fitting. All images were created by rendering the localizations as Gaussians with unit height and a sigma equal to 1 pixel. A pixel intensity value of 600 corresponds to approximately 600 localizations found in or very near the pixel. Images are 9.8 × 9.8 μm and the pixel size is 153 nm.

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