Figure 4
From: New insight into the mechanisms of ectopic fat deposition improvement after bariatric surgery
SG significantly affects insulin signaling. Panels (A–C): Liver Akt Ser473, GSK3αβ Ser21/9, FoxO1 Thr24 phosphorylation was significantly higher in controls than in obese subjects with NAFLD (Akt Ser473: 0.26 ± 0.04 vs 0.96 ± 0.29, P = 0.01; GSK3α Ser21: 1.03 ± 0.18 vs. 3.64 ± 0.97, P = 0.02; GSK3β Ser9: 0.35 ± 0.13 vs. 1.98 ± 0.63, P = 0.02; FoxO1 Thr24: 0.53 ± 0.06 vs. 1.49 ± 017, P = 0.001)). Panels (D–F): Akt Ser473, GSK3αβ Ser21/9 and FoxO1 Thr24 phosphorylation in monocytes is increased after SG (Akt Ser473: 0.27 ± 0.05 vs. 1.21 ± 0.29, P = 0.005; GSK3α Ser21: 0.25 ± 0.08 vs. 0.59 ± 0.20, P = 0.03; GSK3β Ser9: 0.24 ± 0.04 vs. 0.42 ± 0.07, P = 0.01; FoxO1 Thr24: 0.29 ± 0.04 vs. 0.77 ± 0.13, P = 0.001). Panels (G–J): Periodic acid-Schiff staining of liver biopsies (G,H) and monocytes (I,J) of obese subjects with NAFLD before sleeve gastrectomy and controls. Glycogen stores were much higher in controls than in obese subjects. Data are expressed as mean ± SEM (n = 15 obese subjects with NAFLD before and after SG and n = 8 controls).
