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Figure 1

From: Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer

Figure 1

Truncated TALE-FP. (A) Illustration of conventional TALE on DNA and binding domains designed to recognize 7-mer ‘TGTCTGT’ fused with FP. (B) The combined structure of tTALE-FP and DNA. (C) SDS-PAGE for affinity chromatography purification of TALE-eGFP (63 kDa). Purified tTALE-eGFP and tTALE-mCherry. (D) Fluorescence microscopic images and intensity profiles for tTALE-eGFP (2 nM) stained λ DNA molecule (48.5 kb) compared with 2HMG-eGFP34, 2(KW)2-eGFP25, and YOYO-1 stained ones.

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