Figure 2
From: Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer
Salt-dependence of tTALE-FP staining. (A) 1 × TE (Tris-EDTA: 10 mM Tris, 1 mM EDTA, pH 8.0). (B) 1 × PBS (phosphate-buffered saline: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4). (C) λ DNA molecules after the addition of NaCl (10–100 mM) to 1 × TE buffer. Sequence-specific in silico maps of TGTCTGT and AT frequency. Scale bar = 10 μm. (D) λ concatemers in 40 mM NaCl added 1 × TE (i.e., tetramer = 4 × 48.5 kb = 194 kb). All DNA molecules are stained by preincubation in 2 nM tTALE-FP.
