Figure 3
From: Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer
Quantitative analysis with FRET and flow cell with surface-immobilization for the binding affinity of tTALE-eGFP on DNA molecules. (A) Schematics of FRET between tTALE-eGFP and ATTO-590 labeled DNA molecules. (B) Emission spectra from the 30 nM tTALE-eGFP only (dotted line) and the 30/100 nM complex of tTALE-eGFP/5′-ATTO-590 labeled-target sequence (solid line). (C) Fluorescence intensity measurement of tTALE-FP with 1 pM 5′-ATTO-590 labeled-target dsDNA oligomer at 631 nm. Kd = 26.7 nM. (D) Change of fluorescence intensity of 30/100 nM tTALE-eGFP/5′-ATTO-590 labeled dsDNA oligomers at 631 nm with NaCl concentration. •: target DNA. 
: AT-rich DNA. 
: GC-rich DNA. (E) Schematics of the flow cell with surface-immobilization (made in ©BioRender - biorender.com). (F) tTALE-eGFP concentration-dependent fluorescence intensities of λ DNA. (see Materials and Methods for more details).
