Figure 5
From: Inhibitor of Growth 4 (ING4) is a positive regulator of rRNA synthesis
ING4 enhanced H3K9 and histone H4 acetylation. (A) Levels of various modified histones were detected by western blot in three types of HAP1 cells, WT: wild type cells. KO: ING4-KO cells, KO + ING4: the GFP-ING4-rescued ING4-KO cells. Total histone H3 was used as the loading control. (B–D) The relative levels of H3K9ac (B), H3K4me3 (C) and H4ac (D) were evaluated by the densitometric analysis with the results of (A). The results shown are presented means ±SD in three independent experiments. *P < 0.05. (E) Levels of indicated proteins were detected by western blot in siRNA-treated HAP1 cells. siCtrl: cells were transfected with negative control siRNA. siING4: cells were transfected with siRNA targeting ING4 at sequence #1 or #2. Total histone H3 was used as the loading control. (F,G) The relative levels of H3K9ac (F) and H3K4me3 (G) in the western blot performed in (E) were quantified in the same way above. The results shown are presented means ±SD in three independent experiments. *P < 0.05.
