Figure 3
From: FGF Signalling in the Self-Renewal of Colon Cancer Organoids
FGFR-inhibition induces cyst formation and cellular differentiation in pre-established organoids. (A) Cyst induction by FGFR-inhibition in Matrigel-embedded organoids of p8 after 7 d of SU-5402 treatment. (B) Cryo-sections of organoids from patient 3 and (C) patient 4, cultured with CSCM or SU-5402, stained for F-actin and β-catenin. (D) Venn Diagram of genes differentially higher expressed (p < 0.05) in organoids cultured in CSC-Media or (E) after FGFR-inhibition. (F) Significantly enriched GO-terms of biological processes of genes higher expressed after CSC-Media culturing or (G) higher expressed after FGFR-inhibition. Only genes with p < 0.05 and limma-p < 0.05 were considered for GO term-analysis. (H–K) Gene-sets significantly over represented in the CSC-media condition or the FGFR-inhibition condition. NES = normalized enrichment score. (L) Mean fold change (log2) values of single genes belonging to the AP-1 transcription factor complex of Patient 1, 3 and 4 (n = 3). Differential expression values were obtained by microarray analysis; star symbol indicates significance as limma p-value < 0.05. See Table S9 for log2 ratio of single probes detected. (M) Western blot of protein lysates from organoids cultured in CSC-Medium or with SU-5402 (each patient sample, n = 1). Phosphorylated ERK (P-ERK) is compared to total amount of ERK-protein (T-ERK). To increase clarity, blots were cropped. Full-length blots are displayed in Fig. S4.
