Figure 4

RF-EME exposure does not induce elevated 4-hydroxynonenal formation in the testis. Testis sections from untreated control animals (UT), as well as those of the sham and RF-EME exposure groups, were probed with anti-4-hydroxynonenal antibodies (green) to detect by-products of lipid peroxidation. (a) Representative images are depicted, with the scale bar equating to 200 µm. A secondary antibody only control is also included. Corresponding DAPI stained images illustrating tubule morphology are included as insets included in the upper right corner of each panel. (b) Analysis of pixel intensity was performed on the germ cell population within the seminiferous tubules in order to quantify 4-hydroxynonenal expression levels across treatments. Graphical data are presented as mean + SEM (n = 3 mice/treatment group, with 10–20 tubules being analyzed for each testis).