Figure 5

Sperm vitality and motility declines in response to RF-EME exposure. Spermatozoa were collected from the cauda epididymis of untreated control animals (UT), as well as those of the sham and RF-EME exposure groups. (a) Sperm vitality was assessed via the eosin-exclusion method. Next, the percentage of sperm displaying (b) any form of motility, (c) progressive motility, and (d) rapid motility was determined using computer assisted semen analysis. Data are presented as mean + SEM (n = 5–8 mice/treatment group), with a minimum of 100 spermatozoa being analyzed from each animal). The number of biological replicates used is denoted in each bar. *P < 0.05, ***P < 0.001.