Table 1 Number of replicates used for each assay.
Assay/measurement | Untreated control | 1 week | 3 weeks | 5 weeks | |||
|---|---|---|---|---|---|---|---|
Sham | EME | Sham | EME | Sham | EME | ||
Body weight | NA | 20 | 20 | 14 | 14 | 8 | 8 |
Testis histology | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
Testis staining: γH2AX | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
Testis staining: 4-hydroxynonenal | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
Sperm vitality | 5 | 6 | 6 | 5 | 6 | 8 | 8 |
Sperm motility, progressive and rapid | 5 | 5 | 6 | 5 | 6 | 8 | 8 |
Dihydroethidium staining | 4 | 6 | 9 | 5 | 8 | 8 | 8 |
MitoSOX red staining | 6 | 6 | 12 | 5 | 11 | 11 | 11 |
DNA fragmentation | 5 | 6 | 6 | 5 | 6 | 8 | 8 |
Comet tail intensity | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
8OHdG | 3 | 4 | 4 | 4 | 5 | 5 | 5 |
Tyrosine phosphorylation | 3 | NA | NA | NA | NA | 3 | 3 |
Acrosome reaction | 3 | NA | NA | NA | NA | 3 | 3 |
Sperm-zona pellucida adhesion | 3 | NA | NA | NA | NA | 3 | 3 |
Fertilization | 7 | NA | NA | NA | NA | 3 | 5 |
Blastocyst development | 3 | NA | NA | NA | NA | 3 | 3 |
