Figure 1

Bone marrow adipocytes exist within a sinusoidal niche and are predominately unilocular. (a) Representative images of unilocular and multilocular adipocytes located outside the bone, near the periosteal bone surface (periosteum) or within the bone marrow of 3-week-old C57BL/6J mouse femurs and tibias Hematoxylin and eosin images (top) were acquired at 40X, scale bar is 50 µm. Immunofluorescent confocal images (bottom) were acquired at 60X, scale bar is 50 µm. Adipocytes were visualized with α-perilipin antibody (red), sinusoids were visualized with α-endomucin antibody (green), and nuclei were visualized with DAPI (blue). Unilocular and multilocular adipocytes are indicated by white and black arrowheads, respectively. (b) Regional analysis of multilocular adipocyte frequency (relative to total adipocyte number), in 3-week-old and 9-week-old C57BL/6J mouse bones. Metaphysis (Mp), epiphysis (Ep). Mean ± SD. Sample sizes: 3-week n = 4–6, 9-week n = 6–7.