Figure 3

Genetic ablation of Ucp1-Cre expressing cells results in loss of BAT but not BMAT. (a–c) Tissue weights for Ucp1^Cre−/DTA (WT) and Ucp1^Cre+/DTA brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and gonadal white adipose tissue (gWAT). (d) Ucp1^Cre−/DTA and Ucp1^Cre+/DTA mouse total body weight (BW). (e) Ucp1^Cre−/DTA and UCP1^Cre+/DTA tibia length measured by digital caliper. (f–h) Ucp1^Cre−/DTA and Ucp1^Cre+/DTA BAT, iWAT and gWAT tissue weights normalized to total body weight (BW). (i) Representative 3-D images of osmium stained bones to visualize Ucp1^Cre−/DTA (WT) and Ucp1^Cre+/DTA (Ucp1) bone marrow adipose tissue (BMAT). Light grey is outline of bone. Dark grey is osmium-stained BMAT. Analysis and 3-D rendering of the μCT data sets were performed using Scanco software. (j–k) Regional analysis of osmium content in decalcified Ucp1^Cre−/DTA and Ucp1^Cre+/DTA tibias. (j) Proximal BMAT is osmium volume within the proximal epiphysis. (k) Distal BMAT is osmium volume from tibia-fibula junction to distal epiphysis. (l) Osmium content within the whole tibia bone marrow. Mean ± S.D. Two-tailed t-test, *P ≤ 0.05. Sample sizes: Male Cre- = 5–6, Male Cre +  = 4, Female Cre- = 7, Female Cre +  = 7. All ages were 4-weeks old.