Figure 6

BMAT gene enrichment analysis. (a) Gene enrichment and analysis strategy. Of 9,158 detected transcripts, 1,657 were significantly increased in regions of high BMAT (distal tibia and radius/ulna) relative to low BMAT (proximal tibia). (b) Of these, 897 were differentially expressed relative to WAT (blue dots, full dataset in Supp Table 1). (c) Rabbit gene IDs of these differentially expressed transcripts were converted to human identifiers and used for Inguinity Pathway Analysis (full list available in Supp Table 2). (d) Linearized values for osteopontin (Spp1) transcript expression from rabbit whole tissues. 1-way-ANOVA with Tukey’s multiple comparisons test, *p < 0.05. (e) Spp1 transcript from purified inguinal white adipose tissue (iWAT) and tail bone marrow adipocytes (BMAT) normalized to the geometric mean of reference genes Tbp and Ppia. Two-tailed t-test. *p < 0.05. (f) Representative osteopontin immunohistochemistry of human bone and bone marrow. B = bone, Ad = bone marrow adipocytes, white arrows = osteopontin immunostaining in bone (pink), black arrows = osteopontin immunostaining in bone marrow (pink). Scale bar is 50 µm.