Figure 6 | Scientific Reports

Figure 6

From: Specificity and Efficiency of the Uracil DNA Glycosylase-Mediated Strand Cleavage Surveyed on Large Sequence Libraries

Figure 6

Representative sequence motifs for the UDG-mediated uracil cleavage on double- (A,B) and single-stranded (C,D) DNA strands. Substrates were incubated with UDG for different time periods ranging from 5 seconds to 30 minutes (since the cleavage motifs showed little to no sequence dependence, only the 5s, 30s, 60s and 120s were determined for ssDNA). The sequence motifs were extracted from the 1% (41 of 4096 sequences) most cleaved (A,C) and least cleaved (B,D) sequences of the library.

Back to article page