Figure 7
The cleavage efficiency of single-stranded substrates with UDG or USER enzymes. Three parameters are being investigated: linker length (in blue), dU-containing segment length (10, 15 or 20-nt long in black, grey and light grey, respectively) and dU content (from 0 to 100%, on the x axis). All substrates are terminated at the 5ʹ end with a 25mer hybridizable sequence. The corresponding microarrays were treated with either UDG or USER enzyme (5 U, 1 h at 37 °C) and subsequently, in the case of UDG treatment, with EDA/EtOH for 2 h at r.t. The cleavage efficiency corresponds to the loss of hybridization fluorescence after enzymatic treatment and relative to 0%dU controls.
