Figure 1 | Scientific Reports

Figure 1

From: Early pregnancy loss in 15-hydroxyprostaglandin dehydrogenase knockout (15-HPGD−/−) mice due to requirement for embryo 15-HPGD activity

Figure 1

15-HPGD knockout allele completely abrogates 15-HPGD mRNA gene expression and 15-HPGD activity. (A) Wild-type Hpgd+/+, LoxPneo (targeting construct), LoxP(neo−) and Knockout Hpgd−/− alleles. (B) Hpgd−/− mice exhibit a complete absence of 15-HPGD mRNA as analyzed using northern blot of 15-HPGD mRNA in the lung and liver. Cyclophilin mRNA detection is provided as loading control, n = 2 for each genotype. The entire 15-HPGD blot is provided, whilc the cyclophilin blot is truncated for space as the demonstrated differences are qualitative or binary (i.e. positive or negative) rather than quantitative. (C) Hpgd−/− mice completely lack 15-HPGD activity in the liver. Values are mean ± s.e.m. n = 3. (D) Hpgd−/− mice completely lack 15-HPGD activity in the lung. Values are mean ± s.e.m. n = 3.

Back to article page