Figure 5
From: HDAC4 and 5 repression of TBX5 is relieved by protein kinase D1
HDAC4 inhibits MEF2C-TBX5 complex formation on MYH6-promoter target DNA and inhibits its transcriptional activity. (A) 40 bp MYH6 promoter sequence showing conserved TBX5 and MEF2C binding sites that was used for EMSA studies. (B) Electrophoretic mobility shift assay (EMSA) showing the formation of TBX5-MEF2C complex on the MYH6-promoter target DNA (lane 4, black arrowhead). Increasing concentrations of HDAC4 reduced such complex formation (lanes 5–7). (C) Luciferase-reporter assay on the MYH6 promoter indicates that HDAC4 and HDAC5 repress the TBX5-MEF2C transcriptional activity. Results are mean from three individual experiments. Error bars represent SD, *P < 0.02.
