Figure 6
From: HDAC4 and 5 repression of TBX5 is relieved by protein kinase D1
PRKD1 relieves HDAC4 and 5-mediated repression of TBX5. (A) Luciferase-reporter assay showing that CaMKII and CaMKIV can not relieve HDAC4/5-mediated repression of TBX5 on a MYH6 promoter fragment. (B) Luciferase-reporter assay shows that PRKD1 does relieve the HDAC4 and HDAC5-mediated repression of TBX5 on the MYH6 promoter. Results are means from three individual experiments, error bars represent SD, *p < 0.05. (C) Western blot showing the level of PRKD1, CaMKII and CaMKIV proteins used for phosphorylation assays. Full-length blot is shown Supplementary Fig. S4. (D) In vitro phosphorylation activity of PRKD1, CaMKII and CaMKIV on HDAC5 phosphorylation motifs MT1 (S259) and MT2 (S498) (bottom arrowhead). Both motifs were strongly phosphorylated by PRKD1, whereas phosphorylation by CaMKII/CaMKIV was very much weaker.
