Figure 1
Effect of oxidative stress in ARPE-19 cells. Superoxide anions were measured by DHE after 24 h of H2O2 treatment. (A) XTT cell viability as percentage to control. (B) Early apoptotic cells measured using AnnexinV-IP. (C) Flow cytometry histograms: alive cells (annexin V−, PI−), early apoptosis (annexin V+, PI−) and necrotic cells (annexin V+, IP+). (D) Values are expressed as mean ± SEM (n = 3). Statistically significant differences were set at *p < 0.05 and **p < 0.01.
