Figure 5
miRNAs significantly regulated in ARPE-19 cells. Cell miRNAs (A) and sEV miRNA (B) up-regulated in ARPE-19 cells with fold change (FC) > 1. Volcano plot of miRNA expression profile. The X axis represent the miRNA expression level and the Y axis represents p-value (T-student Test). The red lines indicate FC ± 1 P < 0.05 (C,D). Relative expression of the selected Cell miRNAs (E) and sEV miRNAs (F) with FC > 1 (control vs 600 μM H2O2). miRNA expression was quantified by qRT-PCR and calculate using the 2−ΔΔCt. The p-value was calculated by Two-way ANOVA. Results are expressed as mean ± SEM of n = 3–4. *p-value < 0.05 vs Control and ***p-value < 0.001.
