Figure 1
From: High throughput barcoding method for genome-scale phasing
Overview of the phasing technology. High molecular weight DNA fragments are diluted and tagmented with bead-linked transposases. DNA-loaded beads are put into emulsion droplets with barcoding oligonucleotides and primers for amplification, and the constituents of each original molecule is coupled to a unique barcode sequence through emulsion PCR. Following the removal of uncoupled molecules, the library undergoes standard short read sequencing and subsequent grouping of reads according to the barcode sequence. The resulting barcode-linked reads are utilized for long-range DNA phasing, genome-wide haplotyping or reference-free genome assembly.
