Table 4 Normalized ROS levels and difference in ROS levels normalized to day 0, the untreated condition or WT of CLS assay at day 0, 7, 13, and 17 for the ∆sir2 and ∆tor1 strains. Data used in comparison were significant at p < 0.001. Q, PCA and HD referred to quercetin, protocathechuic acid and Hom Dang, respectively. Negative values indicated the decreased values.

Strain	Day	% Normalized ROS levels compared to day 0				% Difference in ROS levels normalized to day 0				% Difference in ROS levels normalized to untreated condition				% Difference in ROS levels normalized to WT
Strain	Day	untreated	Quercetin (Q)	PCA	HD extract	untreated	Quercetin (Q)	PCA	HD extract	untreated	Quercetin (Q)	PCA	HD extract	untreated	Quercetin (Q)	PCA	HD extract
WT	0	100	100	100	100	0	0	0	0	0	−91	−84	−89	0	0	0	0
	3	202	279	319	487	50	64	69	79	0	−88	−75	−73	0	0	0	0
	5	261	356	383	587	62	72	74	83	0	−88	−77	−75	0	0	0	0
	7	294	536	489	705	66	81	80	86	0	−84	−74	−73	0	0	0	0
	11	304	1537	1023	1545	67	93	90	94	0	−56	−48	−43	0	0	0	0
	13	324	2044	1429	1998	69	95	93	95	0	−45	−32	−31	0	0	0	0
	17	340	2653	1685	2293	71	96	94	96	0	−32	−23	−24	0	0	0	0
∆sir2	0	100	100	100	100	0	0	0	0	0	−87	−84	−81	10	63	13	85
	3	219	68	237	256	54	−47	58	61	0	−96	−83	−78	19	−60	−16	−3
	5	277	635	441	407	64	84	77	75	0	−70	−75	−72	17	192	30	28
	7	290	1097	995	802	65	91	90	88	0	−51	−45	−48	8	234	130	110
	11	300	1418	1143	996	67	93	91	90	0	−39	−39	−37	9	51	26	19
	13	301	1609	1435	1216	67	94	93	92	0	−31	−24	−24	2	29	13	13
	17	313	1837	1529	1312	68	95	93	92	0	−24	−22	−21	1	13	3	6
∆tor1	0	100	100	100	100	0	0	0	0	0	−90	−83	−81	12	22	23	94
	3	181	228	260	252	45	56	61	60	0	−88	−75	−73	0	0	0	0
	5	277	1141	554	629	64	91	82	84	0	−61	−66	−56	19	292	78	108
	7	282	1403	883	790	65	93	89	87	0	−53	−47	−45	7	220	122	117
	11	296	1974	1040	951	66	95	90	89	0	−37	−40	−37	9	57	25	19
	13	299	2239	1350	1201	67	96	93	92	0	−29	−23	−22	3	34	16	16
	17	311	2551	1489	1325	68	96	93	92	0	−22	−18	−17	2	18	9	12

Search