Figure 2 | Scientific Reports

Figure 2

From: Identification of Acinetobacter baumannii and its carbapenem-resistant gene blaOXA-23-like by multiple cross displacement amplification combined with lateral flow biosensor

Figure 2

Optimal amplification temperature for pgaD- and blaOXA-23-like-MCDA primer sets. The MCDA amplifications for detection of pgaD (a) and blaOXA-23-like (b) were monitored by real-time measurement of turbidity and the corresponding curves of concentrations of templates were marked in the figures. The threshold value was 0.1 and the turbidity of >0.1 was considered as positive. Five kinetic graphs (1–5) were generated at various temperatures (61 °C-65 °C, 1 °C intervals) with target pathogens DNA. (a) graphs from 2 (62 °C) to 4 (64 °C) showed robust amplification; (b) graphs from 2 (62 °C) to 4 (64 °C) showed robust amplification.

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